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Metodo para el diseño de oligonucleotidos para tecnicas de biologia molecular

La presente divulga un método que puede ser usado para identificar una o un grupo específico de secuencias de adn desde una muestra biológica compleja. Diversos métodos de la biología molecular (ver mas)
La presente divulga un método que puede ser usado para identificar una o un grupo específico de secuencias de adn desde una muestra biológica compleja. Diversos métodos de la biología molecular requieren el uso de secuencias cortas de adn, llamadasoligonucleótidos, que se sintetizan artificialmente a partir de una descripción de las bases que las componen. El método reivindicado permite diseñar oligonucleótidos útiles en dichos procedimientos de biología molecular, tales como el diseño desondas y está caracterizado por la construcción de una base de datos de secuencias de referencia, la selección de un subconjunto de secuencias correspondientes a los organismos de interés, la selección a partir de estas secuencias deoligonucleótidos candidatos, la depuración de estos oligonucleótidos candidatos de acuerdo a criterios de especificidad en la hibridación y de estabilidad termodinámica, y la ordenación de éstos de acuerdo a la especificidad taxonómica. En unsegundo aspecto se protege un método para diseñar pares de oligonucleótidos, los que se requieren en algunas técnicas de biología molecular, tal como la técnica de reacción en cadena de polimerasa (pcr). Esté método es similar al anterior pero seevalúan pares de oligonucleótidos compatibles termodinámicamente que hibridan sobre una misma secuencia a una distancia de un rango determinado

Palabras clave: secuencias cortas de adn pares de oligonucle datos de secuencias tidos secuencias

Titular Vigente: Gonzalez Canales, Mauricio Alejandro 0 %
Martinez Aguilera, Servet 0 %
Biosigma S.a. 100 %
Maass Sepulveda, Alejandro Eduardo 0 %
Aravena Duarte, Andres Octavio 0 %
Parada, Valdec 0 %

Tipo de Solicitud: Patente

Publicacion

Boletin: Fecha: Publicacion:
424 25/07/2007 AR054923A1

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Deposito en:Argentina
Presentacion:16/08/2006
Agente:324 -
Caracter:Independiente

Titular Vigente:

Gonzalez Canales, Mauricio Alejandro 0 %
Martinez Aguilera, Servet 0 %
Biosigma S.a. 100 %
Maass Sepulveda, Alejandro Eduardo 0 %
Aravena Duarte, Andres Octavio 0 %
Parada, Valdec 0 %

Prioridad:

PaisNumeroFecha
CL 2102-2005 17/08/2005

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